Anti-KLHL22 Monoclonal Antibody

Product Name

Anti-KLHL22 Monoclonal Antibody

View all KLHL22 Antibodies

SKU/Catalog Number

A12497

Size

100μl

Form

Liquid

Description

Boster Bio Anti-KLHL22 Monoclonal Antibody catalog # A12497. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KLHL22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12497)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

7B1

Isotype

IgG

Immunogen

Purified recombinant fragment of human KLHL22 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12497 is reactive to KLHL22 in Human

Applications

A12497 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

71.667kDa

Background of KLHL22

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IF, 1:200-1:1000

Validation Images & Assay Conditions

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Western Blot (WB) analysis using KLHL22 Monoclonal antibody against Mouse Brain tissues lysate.

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Immunofluorescence (IF) analysis of U251 cells using KLHL22 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Gene/Protein Information For KLHL22 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ14360; KELCHL; kelch-like 22 (Drosophila); kelch-like protein 22 KLHL22 KELCHL kelch like family member 22 kelch-like protein 22|kelch-like 22

For more info on KLHL22, check out the KLHL22 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KLHL22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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