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- Table of Contents
NRAS antibody (M00099-1) performs well in Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Immunofluorescent analysis of Hela cells, using KRAS+HRAS+NRAS Antibody.
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Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody
NRAS antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, ICC, WB |
Tested Applications
NRAS antibody was positively detected by WB in:
human 293T whole cell, human A431 whole cell, human Jurkat whole cell, human SW620 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NRAS antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IP
FC
Human
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody
View all NRAS Antibodies
View all NRAS Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EBE-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KRAS+HRAS+NRAS
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
21 kDa
Cite This Product
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099-1)
Protocols
Western Blot protocol for NRAS antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NRAS antibody
Publications
Application:
Species:
(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells
Published: 2023-06-14
Journal: CHEMISTRY & BIODIVERSITY
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 37314937
Let-7d microRNA Attenuates 6-OHDA-Induced Injury by Targeting Caspase-3 in MN9D Cells
Published: 2017-10-29
Journal: JOURNAL OF MOLECULAR NEUROSCIENCE
Application: WB
Impact Factor: 2.23
Species: Mouse
PMID: 29082467
Downregulation of miR-21 modulates Ras expression to promote apoptosis and suppress invasion of Laryngeal squamous cell carcinoma
Published: 2010-08-30
Journal: EUROPEAN JOURNAL OF CANCER
Application: WB,IHC
Impact Factor: 4.12
Species: Human
PMID: 20801640
Development of cetuximab-resistant human nasopharyngeal carcinoma cell lines and mechanisms of drug resistance
Published: 2010-04-30
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 2.24
Species: Human
PMID: 20630698
Genistein induces G 2 /M cell cycle arrest via stable activation of ERK1/2 pathway in MDA-MB-231 breast cancer cells
Published: 2008-01-26
Journal: CELL BIOLOGY AND TOXICOLOGY
Application: WB
Impact Factor: 1.76
Species: Human
PMID: 18224451
Identification of effective siRNA against K-ras in human pancreatic cancer cell line MiaPaCa-2 by siRNA expression cassette
Published: 2005-04-07
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IF
Impact Factor: 0.00
Species: Human
PMID: 15801000
Review
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Product Promise
We promise Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00099-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026