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Product Info Summary
| SKU: | M00099-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00099-1
BM4388 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody catalog # M00099-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EBE-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KRAS+HRAS+NRAS
Reactive Species
M00099-1 is reactive to NRAS in Human, Mouse, Rat
Observed Molecular Weight
21 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00099-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human 293T whole cell, human A431 whole cell, human Jurkat whole cell, human SW620 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of KRAS+HRAS+NRAS using anti-KRAS+HRAS+NRAS antibody (M00099-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRAS+HRAS+NRAS antigen affinity purified monoclonal antibody (Catalog # M00099-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRAS+HRAS+NRAS at approximately 21 kDa. The expected band size for KRAS+HRAS+NRAS is at 21 kDa.
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Immunofluorescent analysis of Hela cells, using KRAS+HRAS+NRAS Antibody.
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KRAS+HRAS+NRAS was immunoprecipitated from 1mg of A549 whole cell lysate. A549 whole cell lysate 10ug (Input). The second antibody be without interference from denatured IgG.
Specific Publications For Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (M00099-1)
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1 Customer Q&As for Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody
Question
We received M00099-1 warm and the blue ice has completely melted upon arrival. Could this still work?
Verified customer
Asked: 2020-11-30
Answer
For the Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (M00099-1), you can run a pilot test to see if the antibody could work. We can provide a replacement if the antibody fails to work as expected.
Boster Scientific Support
Answered: 2020-12-01
