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Product Info Summary
| SKU: | A09368 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-KRR1 Antibody
SKU/Catalog Number
A09368
Size
100ul
Form
Liquid
Description
Boster Bio Anti-KRR1 Antibody catalog # A09368. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KRR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09368)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human KRR1(NP_008974.5).
Reactive Species
A09368 is reactive to KRR1 in Human, Mouse, Rat
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09368 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using KRR1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Confocal immunofluorescence analysis of U2OS cells using KRR1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb at dilution of 1:100 . Vimentin for Cytoskeleton staining, DAPI for nuclear staining.
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Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb at dilution of 1:100 . β-Actin for Cytoskeleton staining, DAPI for nuclear staining.
Specific Publications For Anti-KRR1 Antibody (A09368)
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