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Product Info Summary
| SKU: | A14634 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-KV beta.3 Antibody
SKU/Catalog Number
A14634
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KV beta.3 Antibody catalog # A14634. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KV beta.3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14634)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KCNAB3. AA range:293-342
Reactive Species
A14634 is reactive to KCNAB3 in Human, Mouse, Rat
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14634 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using KCNAB3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using KVβ.3 Polyclonal Antibody
Specific Publications For Anti-KV beta.3 Antibody (A14634)
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Customer Q&As
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6 Customer Q&As for Anti-KV beta.3 Antibody
Question
Would anti-KV beta.3 antibody A14634 work on bovine WB with brain?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Our lab technicians have not tested anti-KV beta.3 antibody A14634 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-KV beta.3 antibody A14634 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
Can you help my question with product A14634, anti-KV beta.3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14634 anti-KV beta.3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-25
Question
I was wanting to use your anti-KV beta.3 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
You can see on the product datasheet, A14634 anti-KV beta.3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-02
Question
We are currently using anti-KV beta.3 antibody A14634 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The anti-KV beta.3 antibody (A14634) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-28
Question
I see that the anti-KV beta.3 antibody A14634 works with WB, what is the protocol used to produce the result images on the product page?
Z. Miller
Verified customer
Asked: 2016-07-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-07-06
Question
Is a blocking peptide available for product anti-KV beta.3 antibody (A14634)?
S. Yang
Verified customer
Asked: 2015-09-16
Answer
We do provide the blocking peptide for product anti-KV beta.3 antibody (A14634). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-09-16
