Anti-L3MBTL3 Antibody Picoband™

Product Name

Anti-L3MBTL3 Antibody Picoband™

View all L3MBTL3 Antibodies

SKU/Catalog Number

A10834-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-L3MBTL3 Antibody Picoband™ catalog # A10834-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-L3MBTL3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10834-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human L3MBTL3 recombinant protein (Position: Q11-R686).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10834-1 is reactive to L3MBTL3 in Human

Applications

A10834-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

88.337kDa

Background of L3MBTL3

This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human
Immunohistochemistry, 2-5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of L3MBTL3 using anti-L3MBTL3 antibody (A10834-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L3MBTL3 antigen affinity purified polyclonal antibody (Catalog # A10834-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L3MBTL3 at approximately 90 kDa. The expected band size for L3MBTL3 is at 90 kDa.

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Figure 2. IHC analysis of L3MBTL3 using anti-L3MBTL3 antibody (A10834-1).
L3MBTL3 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-L3MBTL3 Antibody (A10834-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. Flow Cytometry analysis of U251 cells using anti-L3MBTL3 antibody (A10834-1).
Overlay histogram showing U251 cells stained with A10834-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-L3MBTL3 Antibody (A10834-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For L3MBTL3 (Source: Uniprot.org, NCBI)

Alternative Names

H-l(3)mbt-like protein 3; KIAA1798; l(3)mbt-like 3 (Drosophila); L(3)mbt-like protein 3; lethal(3)malignant brain tumor-like protein 3; MBT1; MBT-1; RP11-73O6.1 L3MBTL3 MBT-1, MBT1 L3MBTL histone methyl-lysine binding protein 3 lethal(3)malignant brain tumor-like protein 3|H-l(3)mbt-like protein 3|L3MBTL3, histone methyl-lysine binding protein|L3mbt-like 3|l(3)mbt-like 3

For more info on L3MBTL3, check out the L3MBTL3 Infographic

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In this infographic, you will see the following information for L3MBTL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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