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- Table of Contents
LAG3 antibody (A02869) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of LAG3 in human tonsil tissue with LAG3 antibody at 20 μg/ml.
Red: LAG3 Antibody (A02869)
Blue: DAPI staining
Red: LAG3 Antibody (A02869)
Blue: DAPI staining
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Anti-LAG3 Antibody
LAG3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
LAG3 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-LAG3 Antibody
View all LAG3 Antibodies
View all LAG3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
LAG3 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
LAG3 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3. The immunogen is located within amino acids 110 - 160 of LAG3.
Cross-Reactivity
At least two antibodies are known to exist; this antibody will only detect the largest isoform.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
57.4 kDa
Background of LAG3
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).
Cite This Product
Anti-LAG3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02869)
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Product Promise
We promise Anti-LAG3 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02869
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026