Anti-Laminin alpha-5 LAMA5 Antibody

Product Name

Anti-Laminin alpha-5 LAMA5 Antibody

View all Laminin alpha 5 Antibodies

SKU/Catalog Number

A04624

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Laminin alpha-5 LAMA5 Antibody catalog # A04624. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Laminin alpha-5 LAMA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04624)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Laminin alpha-5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04624 is reactive to LAMA5 in Human, Mouse

Applications

A04624 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

399.737kDa

Background of Laminin alpha 5

Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probaly mediates association of the TREX and CFIm complexes. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.

Xie Y.G., Hum. Mol. Genet. 2:1361-1368(1993).
Nagase T., DNA Res. 6:63-70(1999).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-Laminin alpha-5 LAMA5 Antibody A04624

Western Blot (WB) analysis of RAW HepG2 A549 cells using Laminin alpha-5 polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For LAMA5 (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0533; KIAA1907; laminin alpha-5 chain; laminin subunit alpha-5; laminin, alpha 5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha LAMA5 laminin subunit alpha 5 laminin subunit alpha-5|laminin alpha-5 chain|laminin-10 subunit alpha|laminin-11 subunit alpha|laminin-15 subunit alpha

For more info on LAMA5, check out the LAMA5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LAMA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the LAMA5 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis