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Product Info Summary
| SKU: | A03522-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Laminin Antibody Picoband®
View all LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 Antibodies
SKU/Catalog Number
A03522-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Laminin Antibody Picoband® catalog # A03522-1. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Laminin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03522-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Human fibroblasts derived.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03522-1 is reactive to LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 in Human
Observed Molecular Weight
200 kDa
Calculated molecular weight
337.1 kDa
Background of LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3
Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03522-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue, human HCCT tissue
IHC: human placenta tissue
Validation Images & Assay Conditions
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The effect of MSCs/GDNF transplantation on blood vessels and associated proteins. a - c The images of laminin, CD31, VEGF IHC staining in ipsilateral striata of rats. d - f The numbers of blood vessels, CD31- and VEGF-positive cells in the striata of the different groups ( n = 5 per group). g Representative electron micrographs of brain capillaries in the different groups. The arrowhead indicates a tight junction between two endothelial cells. Asterisks show the basement membrane of the brain capillary. NCe, the nucleus of the endothelial cell; ery, erythrocyte; e, endothelial cell; p, pericyte; a, foot of astrocyte; m, mitochondrion. h Quantitative analysis of the thickness of capillary basement membrane. Scale bar, 100 μm ( a-c ) and 1 μm ( g ). * P < 0.05, *** P < 0.001, and **** P < 0.0001
Index in PubMed under a CC BY license. PMID: 38008847
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Western blot analysis of Laminin using anti-Laminin antibody (A03522-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HCCT tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody (Catalog # A03522-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin at approximately 200 kDa. The expected band size for Laminin is at 177-200 kDa.
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IHC analysis of Laminin using anti-Laminin antibody (A03522-1).
Laminin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Laminin Antibody (A03522-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Laminin Antibody Picoband® (A03522-1)
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