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LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 antibody (A03522-1) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of Laminin using anti-Laminin antibody (A03522-1).
Laminin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Laminin Antibody (A03522-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Laminin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Laminin Antibody (A03522-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Laminin Antibody Picoband®
LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 antibody was positively detected by WB in:
human placenta tissue, human HCCT tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 antibody was positively detected by IHC in:
human placenta tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Laminin Antibody Picoband®
View all LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 Antibodies
View all LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Human fibroblasts derived.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
200 kDa
Calculated Molecular Weight
337.1 kDa
Background of LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3
Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Cite This Product
Anti-Laminin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03522-1)
Protocols
Western Blot protocol for LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 antibody
Immunohistochemistry (IHC) protocol for LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3 antibody
Publications
Application:
Species:
Activation of α7nAChR reduces inflammation and apoptosis, promoting muscle regeneration through the AKT-FOXO1 pathway
Published: 2026-04-03
Journal: CELL DEATH AND DIFFERENTIATION
Application: IF
Impact Factor: 15.40
Species: Mouse
PMID: 41933047
Lycium barbarum polysaccharide mitigates high-fat-diet-induced skeletal muscle atrophy by promoting AMPK/PINK1/Parkin-mediated mitophagy
Published: 2025-01-29
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IF
Impact Factor: 8.50
Species: Mouse
PMID: 39889999
MiR-495 reverses in the mechanical unloading, random rotating and aging induced muscle atrophy via targeting MyoD and inactivating the Myostatin/TGF-β/Smad3 axis
Published: 2024-12-17
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Application: IF
Impact Factor: 3.80
Species: Rat,Mouse
PMID: 39701202
MSCs overexpressing GDNF restores brain structure and neurological function in rats with intracerebral hemorrhage
Published: 2023-11-27
Journal: Molecular Biomedicine
Application: IHC
Impact Factor: 4.00
Species: Rat
PMID: 38008847
Amniotic Membrane Enhances the Characteristics and Function of Stem Cell-Derived Retinal Pigment Epithelium Sheets by Inhibiting the Epithelial–Mesenchymal Transition
Published: 2022-08-03
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 10.63
Species: Human
PMID: 35933105
Mesenchymal Stem Cell-Derived Neuron-Like Cell Transplantation Combined with Electroacupuncture Improves Synaptic Plasticity in Rats with Intracerebral Hemorrhage via mTOR/p70S6K Signaling
Published: 2022-02-15
Journal: Stem Cells International
Application: IHC
Impact Factor: 5.13
Species: Rat
PMID: 35211177
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Product Promise
We promise Anti-Laminin Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A03522-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026