Anti-LAMP2 Antibody Picoband™

Product Name

Anti-LAMP2 Antibody Picoband™

View all LAMP-2/CD107b Antibodies

SKU/Catalog Number

A01573-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LAMP2 Antibody Picoband™ catalog # A01573-3. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LAMP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01573-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human LAMP2 recombinant protein (Position: D128-F410).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01573-3 is reactive to LAMP2 in Human

Applications

A01573-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

44.961kDa

Background of LAMP-2/CD107b

Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (Catalog # A01573-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP2 at approximately 100-110 kDa. The expected band size for LAMP2 is at 45 kDa.

Gene/Protein Information For LAMP2 (Source: Uniprot.org, NCBI)

Alternative Names

CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMPB; LGP110; LGP-96; Lysosomal Associated Membrane Protein 2 ; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2 LAMP2 CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110 lysosomal associated membrane protein 2 lysosome-associated membrane glycoprotein 2|CD107 antigen-like family member B

For more info on LAMP2, check out the LAMP2 Infographic

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In this infographic, you will see the following information for LAMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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