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Product Info Summary
| SKU: | A06802-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-LARG/ARHGEF12 Antibody Picoband™
SKU/Catalog Number
A06802-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LARG/ARHGEF12 Antibody Picoband™ catalog # A06802-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LARG/ARHGEF12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06802-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: T4-S254).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06802-2 is reactive to ARHGEF12 in Human, Rat
Applications
A06802-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
220 kDa
Calculated molecular weight
173.232kDa
Background of LARG
Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LARG/ARHGEF12 using anti-LARG/ARHGEF12 antibody (A06802-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (Catalog # A06802-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The si2093gnal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.
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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-LARG/ARHGEF12 antibody (A06802-2).
Overlay histogram showing HL-60 cells stained with A06802-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LARG/ARHGEF12 Antibody (A06802-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF12 (Source: Uniprot.org, NCBI)
Gene Name
ARHGEF12
Full Name
Rho guanine nucleotide exchange factor 12
Weight
173.232kDa
Alternative Names
ARHGC; ARHGEF12; DKFZp686O2372; KIAA0382PRO2792; LARG; LARGleukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; Rho guanine nucleotide exchange factor (GEF) 12; rho guanine nucleotide exchange factor 12 ARHGEF12 LARG, PRO2792 Rho guanine nucleotide exchange factor 12 rho guanine nucleotide exchange factor 12|Rho guanine nucleotide exchange factor (GEF) 12|leukemia-associated RhoGEF|leukemia-associated rho guanine nucleotide exchange factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGEF12, check out the ARHGEF12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGEF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LARG/ARHGEF12 Antibody Picoband™ (A06802-2)
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