|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-LAT Antibody|
|Description||Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 1(LAT) detection. Tested with WB, IHC-P, ICC in Human.|
|Cite This Product||Anti-LAT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2161)|
|Replacement Item||This antibody may replace the following items: sc-5321|sc-7548|sc-5320|sc-51663|sc-7948|sc-373706|sc-53550|sc-393364|sc-514182 from Santa Cruz Biotechnology.|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human LAT(242-262aa QEAEEVEEEGAPDYENLQELN), different from the related mouse and rat sequences by six amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Linker for activation of T-cells family member 1|
|Molecular Weight||27930 MW|
|Protein Function||Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. .|
|Tissue Specificity||Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). .|
|Subcellular Localization||Cell membrane ; Single-pass type III membrane protein . Present in lipid rafts.|
|Alternative Names||Linker for activation of T-cells family member 1;36 kDa phospho-tyrosine adapter protein;pp36;p36-38;LAT;|
|Research Areas|||immunology|adaptive immunity|t cells|non-cd| signal transduction|adapters|transmembrane|signaling pathway|g protein signaling|small g proteins|ras family||
Background for Linker for activation of T-cells family member 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-LAT Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-LAT Antibody Images
Click the images to enlarge.
IHC(P): Human Appendicitis Tissue
All lanes: Anti LAT (PA2161) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: U937 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,