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- Table of Contents
LDB1 antibody (A01977) performs well in ChIP, ELISA, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot using Boster's affinity purified anti-LDB1 antibody shows detection of LDB1 protein in Jurkat whole cell lysate (W09-001-370). Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Anti-LIM domain-binding protein 1 LDB1 Antibody
LDB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ChIP, ELISA, WB |
Recommend Dilution
Application
Dilution
Species
ELISA: 1:425
000
ChIP: User optimized
WB: 1:500 - 1:3
000
This affinity purified antibody has been tested for use in ELISA
western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene; a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20µg for 10E8 cells for ChIP.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene; a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20µg for 10E8 cells for ChIP.
Product Information
Product Name
Anti-LIM domain-binding protein 1 LDB1 Antibody
View all LDB1 Antibodies
View all LDB1 Antibodies
Form
Liquid (sterile filtered)
Uniprot
Gene ID
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.
Cross-Reactivity
No cross reactivity with other proteins.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
46.5 kDa
Background of LDB1
LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.
Cite This Product
Anti-LIM domain-binding protein 1 LDB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01977)
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Product Promise
We promise Anti-LIM domain-binding protein 1 LDB1 Antibody performs excellently in relevant applications for ChIP, ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01977
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$641.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026