Product Info Summary
SKU: | M00825-1 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LDHA Rabbit Monoclonal Antibody
View all Lactate Dehydrogenase A/LDHA Antibodies
SKU/Catalog Number
M00825-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-LDHA Rabbit Monoclonal Antibody catalog # M00825-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LDHA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00825-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
21L18
Isotype
IgG
Immunogen
A synthesized peptide derived from human LDHA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00825-1 is reactive to LDHA in Human, Mouse, Rat
Applications
M00825-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
36689 MW
Background of Lactate Dehydrogenase A/LDHA
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of LDHA expression in MCF7 cell lysate.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For LDHA (Source: Uniprot.org, NCBI)
Gene Name
LDHA
Full Name
L-lactate dehydrogenase A chain
Weight
36689 MW
Superfamily
LDH/MDH superfamily
Alternative Names
Cell proliferation-inducing gene 19 protein; EC 1.1.1; EC 1.1.1.27; GSD11; Lactate Dehydrogenase A; lactate dehydrogenase M; LDH muscle subunit; LDH1; LDHA; LDH-A; LDHM; LDH-M; L-lactate dehydrogenase A chain; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59 LDHA GSD11, HEL-S-133P, LDHM, PIG19 lactate dehydrogenase A L-lactate dehydrogenase A chain|LDH muscle subunit|LDH-A|LDH-M|cell proliferation-inducing gene 19 protein|epididymis secretory sperm binding protein Li 133P|lactate dehydrogenase M|proliferation-inducing gene 19|renal carcinoma antigen NY-REN-59
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LDHA, check out the LDHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LDHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LDHA Rabbit Monoclonal Antibody (M00825-1)
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