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Product Info Summary
| SKU: | A04957 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LEMD3 Antibody
SKU/Catalog Number
A04957
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LEMD3 Antibody (Catalog # A04957). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LEMD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04957)
Host
Rabbit
Contents
LEMD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LEMD3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LEMD3. The immunogen is located within the last 50 amino acids of LEMD3.
Cross-reactivity
At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2
Reactive Species
A04957 is reactive to LEMD3 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
100.0 kDa
Background of LEMD3
LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04957 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LEMD3 in human colon tissue lysate with LEMD3 antibody at 1 μg/mL.
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Immunohistochemistry of LEMD3 in human colon tissue with LEMD3 antibody at 2.5 μg/mL.
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Immunofluorescence of LEMD3 in human colon tissue with LEMD3 antibody at 5 μg/mL.
Specific Publications For Anti-LEMD3 Antibody (A04957)
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