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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Leucyl tRNA synthetase /LARS Antibody Picoband™
Boster Bio Anti-Leucyl tRNA synthetase /LARS Antibody Picoband™ catalog # A01109. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leucyl tRNA synthetase /LARS Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01109)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human Leucyl tRNA synthetase/LARS recombinant protein (Q628-Q855).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01109 is reactive to LARS in Human, Mouse, Rat
A01109 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LARS using anti-LARS antibody (A01109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARS antigen affinity purified polyclonal antibody (Catalog # A01109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARS at approximately 134KD. The expected band size for LARS is at 134KD.
Gene/Protein Information For LARS (Source: Uniprot.Org, NCBI)
Leucine--tRNA ligase, cytoplasmic
class-I aminoacyl-tRNA synthetase family
Leucine--tRNA ligase, cytoplasmic LARS1 HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, hr025Cl leucyl-tRNA synthetase 1 leucine--tRNA ligase, cytoplasmic|cytoplasmic leucyl-tRNA synthetase|cytosolic leucyl-tRNA synthetase|leucine tRNA ligase 1, cytoplasmic|leucine translase|proliferation-inducing gene 44*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LARS, check out the LARS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LARS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A01109
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