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Product Info Summary
| SKU: | A01109 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Leucyl tRNA synthetase /LARS Antibody Picoband®
SKU/Catalog Number
A01109
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leucyl tRNA synthetase /LARS Antibody Picoband® catalog # A01109. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leucyl tRNA synthetase /LARS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01109)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Leucyl tRNA synthetase/LARS recombinant protein (Q628-Q855).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01109 is reactive to LARS in Human, Mouse, Rat
Observed Molecular Weight
134 kDa
Calculated molecular weight
134.5 kDa
Background of LARS
Leucyl-tRNA synthetase, cytoplasmic is an enzyme that in humans is encoded by the LARS gene. This gene is mapped to 5q32. It encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA (Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01109 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | |
| ELISA | 0.1-0.5μg/ml |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of LARS using anti-LARS antibody (A01109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARS antigen affinity purified polyclonal antibody (Catalog # A01109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARS at approximately 134KD. The expected band size for LARS is at 134KD.
Specific Publications For Anti-Leucyl tRNA synthetase /LARS Antibody Picoband® (A01109)
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5 Customer Q&As for Anti-Leucyl tRNA synthetase /LARS Antibody Picoband®
Question
My question regarding product A01109, anti-Leucyl tRNA synthetase /LARS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01109 anti-Leucyl tRNA synthetase /LARS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
I see that the anti-Leucyl tRNA synthetase /LARS antibody A01109 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-21
Question
We are currently using anti-Leucyl tRNA synthetase /LARS antibody A01109 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
A. Jackson
Verified customer
Asked: 2016-04-25
Answer
The anti-Leucyl tRNA synthetase /LARS antibody (A01109) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-25
Question
Does anti-Leucyl tRNA synthetase /LARS antibody A01109 work on pig ELISA with hippocampus?
V. Jones
Verified customer
Asked: 2015-03-30
Answer
Our lab technicians have not validated anti-Leucyl tRNA synthetase /LARS antibody A01109 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Leucyl tRNA synthetase /LARS antibody A01109 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig hippocampus in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-30
Question
I was wanting to use your anti-Leucyl tRNA synthetase /LARS antibody for WB for rat erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat erythroleukemia identification?
J. Li
Verified customer
Asked: 2014-05-02
Answer
It shows on the product datasheet, A01109 anti-Leucyl tRNA synthetase /LARS antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-05-02
