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Product Info Summary
| SKU: | A13256 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LGI2 Antibody
SKU/Catalog Number
A13256
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LGI2 Antibody (Catalog # A13256). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LGI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LGI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13256)
Host
Rabbit
Contents
LGI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LGI2 antibody was raised against a 11 amino acid synthetic peptide from near the amino terminus of human LGI2. The immunogen is located within the first 50 amino acids of LGI2.
Cross-reactivity
This LGI2 antibody is predicted to be specific to LGI2 and not recognize other LGI proteins. Three isoforms of LGI2 are known to exist; this LGI2 antibody will recognize only the longest.
Reactive Species
A13256 is reactive to LGI2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
63.0 kDa
Background of LGI2
The leucine-rich, glioma inactivated gene 2 (LGI2) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI2 have not been found to be associated with ADTLE.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13256 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LGI2 antibody can be used for the detection of LGI2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LGI2 in 293 cell lysate with LGI2 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-LGI2 Antibody (A13256)
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