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Product Info Summary
| SKU: | A13999 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-LIAR ANKRD54 Antibody
SKU/Catalog Number
A13999
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LIAR ANKRD54 Antibody (Catalog # A13999). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LIAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LIAR ANKRD54 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13999)
Host
Rabbit
Contents
LIAR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LIAR antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LIAR. The immunogen is located within amino acids 230 - 280 of LIAR.
Reactive Species
A13999 is reactive to ANKRD54 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.5 kDa
Background of ANKRD54
Ankyrin (ANK) repeats mediate protein-protein interactions in diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20. ANK repeats may occur in combinations with other types of domains. The structural repeat unit contains two anti-parallel helices and a beta-hairpin, with repeats stacked in a superhelical arrangement. LIAR, also known as ANKRD54, is a recently identified ANK repeat-containing protein that is predominantly expressed in tissues rich in cilliated cells, such as olfactory sensory neurons and is predicted to be important to cilia. At least three isoforms of LIAR are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13999 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LIAR antibody can be used for detection of LIAR by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LIAR in mouse kidney tissue lysate with LIAR antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of LIAR in Mouse Kidney cells with LIAR antibody at 20 μg/mL.
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Immunohistochemistry of LIAR in mouse kidney tissue with LIAR antibody at 2.5 μg/mL.
Specific Publications For Anti-LIAR ANKRD54 Antibody (A13999)
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