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Product Info Summary
| SKU: | M13360 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-LIME1 Purified Monoclonal Antibody
SKU/Catalog Number
M13360
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LIME1 Purified Monoclonal Antibody (Catalog# M13360). Tested in WB, IHC-P, FCM application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LIME1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13360)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
LIME-10
Isotype
Mouse IgG2a
Immunogen
COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin. The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M13360 is reactive to LIME1 in Human
Observed Molecular Weight
30 kDa
Calculated molecular weight
31.3 kDa
Background of LIME1
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13360 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.
Western blotting: Reducing conditions; recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Validation Images & Assay Conditions
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Anti-Hu LIME1 purified antibody (clone LIME-10) specificity verification by flow cytometry
Analysis of the antibody staining was performed on fixed and permeabilized (eBioscience™ Intracellular Fixation & Permeabilization Buffer Set, Thermofisher) cell suspension.
Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 µg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel).
Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 µg/ml, black-dashed histogram).
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Western blotting analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.
Specific Publications For Anti-LIME1 Purified Monoclonal Antibody (M13360)
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