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Product Info Summary
| SKU: | A01273 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-LIS1 PAFAH1B1 Antibody
SKU/Catalog Number
A01273
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LIS1 PAFAH1B1 Antibody (Catalog # A01273). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LIS1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LIS1 PAFAH1B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01273)
Host
Rabbit
Contents
LIS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1. The immunogen is located within amino acids 340 - 390 of LIS1.
Reactive Species
A01273 is reactive to PAFAH1B1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.6 kDa
Background of PAFAH1B1
Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01273 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 μg/mL.
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Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 μg/mL.
Specific Publications For Anti-LIS1 PAFAH1B1 Antibody (A01273)
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