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Product Info Summary
| SKU: | A02577-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Livin/Birc7 Antibody Picoband®
SKU/Catalog Number
A02577-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Livin/Birc7 Antibody Picoband® catalog # A02577-2. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Livin/Birc7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02577-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Livin/Birc7 recombinant protein (Position: Q41-S285).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02577-2 is reactive to Birc7 in Mouse
Observed Molecular Weight
33 kDa
Calculated molecular weight
32.0 kDa
Background of Birc7
Baculoviral IAP repeat-containing protein 7 is a protein that in humans is encoded by the BIRC7 gene. This protein is a member of the family of inhibitor of apoptosis proteins(IAP) and contains a single copy of a baculovirus IAP repeat(BIR) and a RING finger motif. Its gene is mapped to 20q13.3. It has got 280- and 298- amino acids. The protein is highly expressed in melanomas while lowly expressed in some lymphomas, fetal kidney, fetal liver, testis and thymus. BIRC7 plays a vital role in blocking apoptosis induced.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02577-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse heart tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of Livin/Birc7 using anti-Livin/Birc7 antibody (A02577-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Livin/Birc7 antigen affinity purified polyclonal antibody (Catalog # A02577-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Livin/Birc7 at approximately 33 kDa. The expected band size for Livin/Birc7 is at 33 kDa.
Specific Publications For Anti-Livin/Birc7 Antibody Picoband® (A02577-2)
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