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Product Info Summary
| SKU: | A14796 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LONRF1 Antibody Picoband®
SKU/Catalog Number
A14796
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LONRF1 Antibody Picoband® catalog # A14796. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LONRF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14796)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human LONRF1 recombinant protein (Position: L52-D442).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A14796 is reactive to LONRF1 in Human
Observed Molecular Weight
100 kDa
Calculated molecular weight
86.7 kDa
Background of LONRF1
LONRF1, also known as LON peptidase N-terminal domain and RING finger protein 1, consists of an 86.7 kDa and an 85.5 kDa isoform. There is no information regarding the function of the protein or the research being conducted with the protein. The protein interacts with TRIM9, UBE2L6, and BRCA1 proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14796 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of LONRF1 using anti-LONRF1 antibody (A14796).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONRF1 antigen affinity purified polyclonal antibody (Catalog # A14796) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONRF1 at approximately 100 kDa. The expected band size for LONRF1 is at 87 kDa.
Specific Publications For Anti-LONRF1 Antibody Picoband® (A14796)
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