Click image to see more details
Product Info Summary
| SKU: | A30833 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-LPHN2 ADGRL2 Antibody
SKU/Catalog Number
A30833
Size
100ul
Form
Liquid
Description
Boster Bio Anti-LPHN2 ADGRL2 Antibody (Catalog# A30833). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LPHN2 ADGRL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30833)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human LPHN2. Immunogen sequence location: 551 - 600
Reactive Species
A30833 is reactive to ADGRL2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
163.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30833 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of COS7 cells, using LPHN2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using LPHN2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-LPHN2 ADGRL2 Antibody (A30833)
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Customer Q&As
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3 Customer Q&As for Anti-LPHN2 ADGRL2 Antibody
Question
Does anti-LPHN2 antibody A30833 work on bovine IHC with liver?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Our lab technicians have not validated anti-LPHN2 antibody A30833 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-LPHN2 antibody A30833 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-LPHN2 antibody A30833 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
The anti-LPHN2 antibody (A30833) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-15
Question
See attached the WB image, lot number and protocol we used for liver using anti-LPHN2 antibody A30833. Please let me know if you require anything else.
Z. Zhang
Verified customer
Asked: 2017-05-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-19
