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Product Info Summary
| SKU: | M02463 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody
SKU/Catalog Number
M02463
BM3907 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody catalog # M02463. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02463)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Lrp2 / Megalin
Reactive Species
M02463 is reactive to LRP2 in Human, Mouse, Rat
Observed Molecular Weight
280 kDa
Calculated molecular weight
522.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02463 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of Lrp2 expression in 293T cell lysate.
Specific Publications For Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (M02463)
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Customer Q&As
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7 Customer Q&As for Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody
Question
What is the recommended storage of M02463?
Verified customer
Asked: 2022-06-07
Answer
The Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (M02463) can be stored at -20° C for up to 2 years.
Boster Scientific Support
Answered: 2022-06-07
Question
I see that the anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-10
Question
We are currently using anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-Lrp2 / Megalin Rabbit Monoclonal antibody (M02463) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
Does anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 work on zebrafish WB with cortex of kidney?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Our lab technicians have not tested anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Lrp2 / Megalin Rabbit Monoclonal antibody M02463 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cortex of kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-25
Question
What kind of running buffer was used for M02463?
Verified customer
Asked: 2019-08-23
Answer
Our lab used AR0139 as running buffer for the Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody (M02463). https://www.bosterbio.com/tris-glycine-sds-running-buffer-pack-ar0139-boster.html
Boster Scientific Support
Answered: 2019-08-23
Question
Has lysate0032 been tested to express LRP2 by using M02463 antibody?
Verified customer
Asked: 2019-07-18
Answer
Yes, our lab technicians confirmed that the Human 293T Whole Cell Lysate LYSATE0032 expressed LRP2 by using Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody M02463 during our test.
Boster Scientific Support
Answered: 2019-07-18
Question
We ordered #M02463 Anti-Lrp2 / Megalin Rabbit Monoclonal Antibody, last week. Please let us ask you about your Rabbit monoclonal antibodies. 1. Are your rabbit monoclonal antibodies recombinant antibodies? What kind of host cells was used to express each recombinant antibody? 2. How was the transgene, the variable region gene, introduced into the host cells? Plasmid transfection, viral infection or any other way?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
Please see the following answers for your questions. 1. Are your rabbit monoclonal antibodies recombinant antibodies? What kind of host cells was used to express each recombinant antibody? M02463 is a recombinant protein. Host cell is 293 eucaryote cell. 2. How was the transgene, the variable region gene, introduced into the host cells? Plasmid transfection, viral infection or any other way? This information is confidential. Sorry we can not disclose it.
Boster Scientific Support
Answered: 2019-07-05
