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LRR1 antibody (A11958) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of LRR1 using anti-LRR1 antibody (A11958).
LRR1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LRR1 Antibody (A11958) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
LRR1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LRR1 Antibody (A11958) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-LRR1 Antibody Picoband®
LRR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
LRR1 antibody was positively detected by WB in:
human U2OS whole cell, human A549 whole cell, human PC-3 whole cell, human MCF-7 whole cell, A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LRR1 antibody was positively detected by IHC in:
human brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LRR1 antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-LRR1 Antibody Picoband®
View all LRR1 Antibodies
View all LRR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LRR1.(DNLGGTEAPIISYFCSLGC).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
47 kDa
Calculated Molecular Weight
46.7 kDa
Background of LRR1
The protein encoded by this gene contains a leucine-rich repeat (LRR). It specifically interacts with TNFRSF9/4-1BB, a member of the tumor necrosis factor receptor (TNFR) superfamily. Overexpression of this gene suppresses the activation of NF-kappa B induced by TNFRSF9 or TNF receptor-associated factor 2 (TRAF2), which suggests that this protein is a negative regulator of TNFRSF9-mediated signaling cascades. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-LRR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11958)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
89%
Moderate Homology
Cow
89%
Moderate Homology
Monkey
89%
Moderate Homology
Sheep
84%
Moderate Homology
Dog
84%
Moderate Homology
Rat
74%
Moderate Homology
Mouse
68%
Low homology
Zebrafish
68%
Low homology
Drosophila
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LRR1 antibody
Immunohistochemistry (IHC) protocol for LRR1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for LRR1 antibody
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Product Promise
We promise Anti-LRR1 Antibody Picoband® performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A11958
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026