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Product Info Summary
| SKU: | M08071 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-LSD2 / AOF1 Monoclonal Antibody
SKU/Catalog Number
M08071
BM5366 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LSD2 / AOF1 Monoclonal Antibody catalog # M08071. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LSD2 / AOF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08071)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADAG-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LSD2 / AOF1 Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Reactive Species
M08071 is reactive to KDM1B in Human, Mouse, Rat
Observed Molecular Weight
93 kDa
Calculated molecular weight
92.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08071 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human RT4 whole cell, HeLa cell, RAW2647 cell, PC12 cell
Validation Images & Assay Conditions
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Western blot analysis of KDM1B using anti-KDM1B antibody (M08071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM1B antigen affinity purified monoclonal antibody (Catalog # M08071) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM1B at approximately 93 kDa. The expected band size for KDM1B is at 93 kDa.
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Western blot analysis of LSD2 / AOF1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate.
Specific Publications For Anti-LSD2 / AOF1 Monoclonal Antibody (M08071)
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3 Customer Q&As for Anti-LSD2 / AOF1 Monoclonal Antibody
Question
My question regarding product M08071, anti-LSD2 / AOF1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08071 anti-LSD2 / AOF1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-23
Question
We are currently using anti-LSD2 / AOF1 Monoclonal antibody M08071 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
The anti-LSD2 / AOF1 Monoclonal antibody (M08071) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-19
Question
We are interested in to test anti-LSD2 / AOF1 Monoclonal antibody M08071 on human cervix for research purposes, then I may be interested in using anti-LSD2 / AOF1 Monoclonal antibody M08071 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Yang
Verified customer
Asked: 2015-09-09
Answer
The products we sell, including anti-LSD2 / AOF1 Monoclonal antibody M08071, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-09
