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- Table of Contents
LZIC antibody (A15087) performs well in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of 293T cells using anti-LZIC antibody (A15087).
Overlay histogram showing 293T cells stained with A15087 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LZIC Antibody (A15087, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing 293T cells stained with A15087 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LZIC Antibody (A15087, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Designing an IHC experiment for LZIC? The guide covers the expected staining pattern, antigen retrieval, antibodies backed by real IHC images, tissue controls, and protocols taken from published papers.
Open the LZIC IHC GuideBundle Save
Anti-LZIC Antibody Picoband®
LZIC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
LZIC antibody was positively detected by WB in:
human PC-3 whole cell, human HepG2 whole cell, human 293T whole cell, human K562 whole cell, human HEL whole cell, human Jurkat whole cell, rat brain tissue, rat thymus tissue, mouse brain tissue, mouse thymus tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LZIC antibody was positively detected by IHC in:
human prostatic cancer tissue, mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LZIC antibody was positively detected by ICC/IF in:
A549 cell
LZIC antibody was positively detected by IP in:
PC-3 cell
LZIC antibody was positively detected by FC in:
293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-LZIC Antibody Picoband®
View all LZIC Antibodies
View all LZIC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derivedhumanLZICrecombinantprotein(Position:N67-K187).HumanLZICshares91.7%and95%aminoacid(aa)sequenceidentitywithmouseandratLZIC,respectively.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
21.5 kDa
Background of LZIC
LZIC is closely related to ICAT, a physiological inhibitor ofthe Wnt pathway that interacts physically and functionally withβ-catenin to prevent the transcription ofWnt target genes. LZIC'sICAT-homologousregion is highly similarto ICAT with particular conservation ofresiduesthat are used by ICAT forβ-catenin-binding. LZIC does not interactwithβ-catenin in vitro orin vivo. LZIC is a protein conserved in vertebrates, isrequired for neuronal survival.
Cite This Product
Anti-LZIC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15087)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Sheep
98%
High Homology
Dog
98%
High Homology
Cow
98%
High Homology
Rat
95%
High Homology
Rabbit
95%
High Homology
Mouse
92%
High Homology
Zebrafish
84%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LZIC antibody
Immunohistochemistry (IHC) protocol for LZIC antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for LZIC antibody
Immunoprecipitation (IP) protocol for LZIC antibody
Flow Cytometry (FC) protocol for LZIC antibody
Publications
Application:
Species:
Macrophage-derived exosome as bioengineered nanotherapeutics for silica-induced reproductive dysregulation
Published: 2026-02-14
Journal: JOURNAL OF HAZARDOUS MATERIALS
Application: IF,WB
Impact Factor: 11.30
Species: Mouse
PMID: 41702166
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Product Promise
We promise Anti-LZIC Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A15087
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026