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Product Info Summary
| SKU: | A05678 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-M-RIP MPRIP Antibody
SKU/Catalog Number
A05678
Size
100μl
Form
Liquid
Description
Boster Bio Anti-M-RIP MPRIP Antibody catalog # A05678. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-M-RIP MPRIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05678)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human M-RIP.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05678 is reactive to MPRIP in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
116.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05678 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of 293 cells using M-RIP Polyclonal Antibody
Specific Publications For Anti-M-RIP MPRIP Antibody (A05678)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-M-RIP MPRIP Antibody
Question
Is a blocking peptide available for product anti-M-RIP antibody (A05678)?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We do provide the blocking peptide for product anti-M-RIP antibody (A05678). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-M-RIP antibody A05678 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-M-RIP antibody (A05678) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
Is this A05678 anti-M-RIP antibody reactive to the isotypes of MPRIP?
Verified Customer
Verified customer
Asked: 2019-02-13
Answer
The immunogen of A05678 anti-M-RIP antibody is Synthesized peptide derived from the Internal region of human M-RIP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-13
Question
Does A05678 anti-M-RIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Collins
Verified customer
Asked: 2014-08-22
Answer
It shows on the product datasheet, A05678 anti-M-RIP antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-22
Question
I see that the anti-M-RIP antibody A05678 works with WB, what is the protocol used to produce the result images on the product page?
Z. Wu
Verified customer
Asked: 2013-06-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-06-14
