Anti-MAFG Antibody Picoband™

Product Name

Anti-MAFG Antibody Picoband™

View all MAFG Antibodies

SKU/Catalog Number

A04636-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MAFG Antibody Picoband™ catalog # A04636-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MAFG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04636-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MAFG, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04636-2 is reactive to MAFG in Human

Applications

A04636-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

18 kDa

Calculated molecular weight

17.85kDa

Background of MAFG

Transcription factor MafG is a bZip Maf transcription factor protein that in humans is encoded by the MAFG gene. Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MAFG using anti-MAFG antibody (A04636-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFG antigen affinity purified polyclonal antibody (Catalog # A04636-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFG at approximately 18 kDa. The expected band size for MAFG is at 18 kDa.

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Figure 2. Flow Cytometry analysis of A431 cells using anti-MAFG antibody (A04636-2).
Overlay histogram showing A431 cells stained with A04636-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAFG Antibody (A04636-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For MAFG (Source: Uniprot.org, NCBI)

Alternative Names

basic leucine zipper transcription factor MafG; hMAF; MafG; MGC13090; MGC20149; transcription factor MafG; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G; v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog G MAFG hMAF MAF bZIP transcription factor G transcription factor MafG|basic leucine zipper transcription factor MafG|v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G

For more info on MAFG, check out the MAFG Infographic

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In this infographic, you will see the following information for MAFG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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