Anti-MAGE-A5 Antibody

MAGEA5 antibody

Boster Bio Anti-MAGE-A5 Antibody catalog # A17180. Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A17180
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-MAGE-A5 Antibody

View all MAGEA5 Antibodies

SKU/Catalog Number

A17180

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MAGE-A5 Antibody catalog # A17180. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAGE-A5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17180)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1B9C1

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human MAGE-A5. at AA rangle: 40-120

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A17180 is reactive to MAGEA5 in Human

Applications

A17180 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

13.016kDa

Background of MAGEA5

BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MAGEA5 (Source: Uniprot.org, NCBI)

Gene Name

MAGEA5

Full Name

Melanoma-associated antigen 5

Weight

13.016kDa

Alternative Names

CT1.5; MAGE5; MAGEA4; MAGEA5 melanoma antigen family A, 5 MAGEA5P CT1.5, MAGE5, MAGEA4, MAGEA5 MAGE family member A5, pseudogene MAGE family member A5|MAGE-5 antigen|MAGE-5a antigen|MAGE-5b antigen|cancer/testis antigen 1.5|cancer/testis antigen family 1, member 5|melanoma antigen family A, 5|melanoma antigen family A5|melanoma-associated antigen 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAGEA5, check out the MAGEA5 Infographic

MAGEA5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAGEA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A17180 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Transplantation of rat frozen-thawed testicular tissues: Does fragment size matter?

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7 Customer Q&As for Anti-MAGE-A5 Antibody

Question

Is this A17180 anti-MAGE-A5 antibody reactive to the isotypes of MAGEA5?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

The immunogen of A17180 anti-MAGE-A5 antibody is Synthesized peptide derived from the Internal region of human MAGE-A5. at AA rangle: 40-120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-18

Question

Is a blocking peptide available for product anti-MAGE-A5 antibody (A17180)?

G. Miller

Verified customer

Asked: 2019-05-23

Answer

We do provide the blocking peptide for product anti-MAGE-A5 antibody (A17180). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-23

Question

We are currently using anti-MAGE-A5 antibody A17180 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The anti-MAGE-A5 antibody (A17180) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-09

Question

Do you have a BSA free version of anti-MAGE-A5 antibody A17180 available?

R. Parker

Verified customer

Asked: 2018-08-06

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAGE-A5 antibody A17180 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-06

Question

Can you help my question with product A17180, anti-MAGE-A5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

O. Williams

Verified customer

Asked: 2017-11-08

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17180 anti-MAGE-A5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-11-08

Question

I see that the anti-MAGE-A5 antibody A17180 works with WB, what is the protocol used to produce the result images on the product page?

W. Jackson

Verified customer

Asked: 2017-10-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-10-13

Question

Would A17180 anti-MAGE-A5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

It shows on the product datasheet, A17180 anti-MAGE-A5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-21

Order DetailsPrice
A17180

100uL

$399

Free Secondary Antibody

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A17180
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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