Anti-MAML3 (Mastermind Like Transcriptional Coactivator 3) Monoclonal Antibody

Product Name

Anti-MAML3 (Mastermind Like Transcriptional Coactivator 3) Monoclonal Antibody

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SKU/Catalog Number

M07975

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MAML3 (Mastermind Like Transcriptional Coactivator 3) Monoclonal Antibody (Catalog # M07975). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MAML3 (Mastermind Like Transcriptional Coactivator 3) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07975)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: MAML3/1303

Isotype

IgG1, kappa

Immunogen

Recombinant human MAML3 protein fragment

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M07975 is reactive to MAML3 in Human

Applications

M07975 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

122.293kDa

Background of MAML3

MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Pancreas stained with Anti-MAML3 Monoclonal Antibody (MAML3/1303).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-MAML3 Monoclonal Antibody (MAML3/1303).

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Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Anti-MAML3 Monoclonal Antibody (MAML3/1303).

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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-MAML3 Monoclonal Antibody (MAML3/1303).

Gene/Protein Information For MAML3 (Source: Uniprot.org, NCBI)

Alternative Names

CAG/CTG 3; ERDA3; MAM-2; mam-3; mastermind-like 3 (Drosophila); mastermind-like protein 3; polyglutamine rich; trinucleotide repeat containing 3 MAML3 CAGH3, ERDA3, GDN, MAM-2, MAM2, TNRC3, mam-3 mastermind like transcriptional coactivator 3 mastermind-like protein 3|CAG repeat containing (glia-derived nexin I alpha)|expanded repeat domain, CAG/CTG 3|polyglutamine rich|trinucleotide repeat containing 3

For more info on MAML3, check out the MAML3 Infographic

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In this infographic, you will see the following information for MAML3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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