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- Table of Contents
MANSC4 antibody (A19343) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Caco-2 cells using anti-MANSC4 antibody (A19343).
Overlay histogram showing Caco-2 cells stained with A19343 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MANSC4 Antibody (A19343, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing Caco-2 cells stained with A19343 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MANSC4 Antibody (A19343, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-MANSC4 Antibody Picoband®
MANSC4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
MANSC4 antibody was positively detected by WB in:
human PC-3 whole cell, human U2OS whole cell, monkey COS-7 whole cell, rat kidney tissue, mouse kidney tissuemouse HBZY whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MANSC4 antibody was positively detected by IHC in:
human liver cancer tissue, human testicular seminoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MANSC4 antibody was positively detected by IF in:
human liver cancer tissue
MANSC4 antibody was positively detected by FC in:
Caco-2 cell, PC-3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MANSC4 Antibody Picoband®
View all MANSC4 Antibodies
View all MANSC4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human MANSC4 recombinant protein (Position: R36-R140). Human MANSC4 shares 83% amino acid (aa) sequence identity with mouse MANSC4.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
37.9 kDa
Background of MANSC4
MANSC4 is predicted to be integral component of membrane.
Cite This Product
Anti-MANSC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19343)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Cow
91%
High Homology
Rat
88%
Moderate Homology
Rabbit
85%
Moderate Homology
Mouse
84%
Moderate Homology
Zebrafish
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MANSC4 antibody
Immunohistochemistry (IHC) protocol for MANSC4 antibody
Immunofluorescence (IF) protocol for MANSC4 antibody
Flow Cytometry (FC) protocol for MANSC4 antibody
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Product Promise
We promise Anti-MANSC4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A19343
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026