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Product Info Summary
| SKU: | M01543-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, IHC-P-Leica |
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Product info
Product Name
Anti-MAP1LC3A antibody(N-term)
SKU/Catalog Number
M01543-2
Size
50 µl
Description
Boster Bio Anti-MAP1LC3A antibody(N-term) (Catalog # M01543-2). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MAP1LC3A antibody(N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01543-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-40 amino acids from human.
Reactive Species
M01543-2 is reactive to MAP1LC3A in Human, Mouse, Rat
Calculated molecular weight
14.3 kDa
Background of MAP1LC3A
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01543-2 is guaranteed for WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
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All lanes : Anti-MAP1LC3A antibody (N-term) at 1:500 dilution
Lane 1: Human brain tissue lysate
Lane 2: U-251 MG whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-MAP1LC3A antibody(N-term) at 1:2000 dilution
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using M01543-2 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human heart tissue using M01543-2 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-MAP1LC3A antibody(N-term) (M01543-2)
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