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Product Info Summary
| SKU: | M01543 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody
SKU/Catalog Number
M01543
BM4607 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody catalog # M01543. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01543)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GCH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MAP1LC3A
Reactive Species
M01543 is reactive to MAP1LC3A in Human, Mouse, Rat
Observed Molecular Weight
14 kDa
Calculated molecular weight
14.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01543 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: human testis tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of MAP1LC3A expression in Human brain lysate.
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Immunohistochemical analysis of paraffin-embedded human testis, using MAP1LC3A Antibody.
Specific Publications For Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody (M01543)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for uterus using anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody M01543. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-02
Question
Do you have a BSA free version of anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody M01543 available?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody M01543 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-07
Question
I have a question about product M01543, anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Wu
Verified customer
Asked: 2019-05-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-31
Question
Would M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
You can see on the product datasheet, M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-12
Question
I was wanting to use your anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody for IP for human uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human uterus identification?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
As indicated on the product datasheet, M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-21
