Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody

LC3A antibody

Boster Bio Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody catalog # M01543. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01543
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody

View all LC3A Antibodies

SKU/Catalog Number

M01543

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody catalog # M01543. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01543)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GCH-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MAP1LC3A

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01543 is reactive to Map1lc3a in Human, Mouse, Rat

Applications

M01543 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

14.272kDa

Background of LC3A

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For Map1lc3a (Source: Uniprot.org, NCBI)

Gene Name

Map1lc3a

Full Name

Microtubule-associated proteins 1A/1B light chain 3A

Weight

14.272kDa

Superfamily

ATG8 family

Alternative Names

Apg8; APG8a; Apg8p3; ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; lc3-i, ii; MAP1A/1B light chain 3 A; MAP1ALC3; MAP1ALC3MAP1A/MAP1B LC3 A; MAP1BLC3MAP1A/MAP1B light chain 3 A; MAP1LC3A; microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; MLP3A Map1lc3a|1010001H21Rik, 4922501H04Rik, LC, LC3, LC3a|microtubule-associated protein 1 light chain 3 alpha|microtubule-associated proteins 1A/1B light chain 3A|MAP1 light chain 3-like protein 1|MAP1A/MAP1B LC3 A|MAP1A/MAP1B light chain 3 A|autophagy-related protein LC3 A|autophagy-related ubiquitin-like modifier LC3 A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Map1lc3a, check out the Map1lc3a Infographic

Map1lc3a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Map1lc3a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody

Question

See below the WB image, lot number and protocol we used for uterus using anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody M01543. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-02

Question

Do you have a BSA free version of anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody M01543 available?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody M01543 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-07

Question

I have a question about product M01543, anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Wu

Verified customer

Asked: 2019-05-31

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-31

Question

Would M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-12

Answer

You can see on the product datasheet, M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-12

Question

I was wanting to use your anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody for IP for human uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human uterus identification?

Verified Customer

Verified customer

Asked: 2018-05-21

Answer

As indicated on the product datasheet, M01543 anti-MAP1LC3A/Lc3A Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-21

Order DetailsPrice
M01543

100μL

$370
M01543-30ul

30μL sample

$99
M01543-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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