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Product Info Summary
| SKU: | A00902-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MAP3K20 Antibody Picoband®
SKU/Catalog Number
A00902-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAP3K20 Antibody Picoband® catalog # A00902-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP3K20 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00902-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MAP3K20 recombinant protein (Position: F276-E511).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00902-1 is reactive to MAP3K20 in Human, Mouse, Rat
Observed Molecular Weight
91 kDa
Calculated molecular weight
91.2 kDa
Background of MAP3K20
Sterile alpha motif and leucine zipper containing kinase AZK, also known as ZAK, is a human gene. This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00902-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse thymus tissue, human PANC-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MAP3K20 using anti-MAP3K20 antibody (A00902-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody (Catalog # A00902-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
Click image to see more details
Western blot analysis of MAP3K20 using anti-MAP3K20 antibody (A00902-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K20 antigen affinity purified polyclonal antibody (Catalog # A00902-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K20 at approximately 91KD. The expected band size for MAP3K20 is at 91KD.
Specific Publications For Anti-MAP3K20 Antibody Picoband® (A00902-1)
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4 Customer Q&As for Anti-MAP3K20 Antibody Picoband®
Question
Will anti-MAP3K20 antibody A00902-1 work on primate WB with embl:bad92211.1}?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
Our lab technicians have not validated anti-MAP3K20 antibody A00902-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MAP3K20 antibody A00902-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate embl:bad92211.1} in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
Do you have a BSA free version of anti-MAP3K20 antibody A00902-1 available?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MAP3K20 antibody A00902-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-30
Question
We are currently using anti-MAP3K20 antibody A00902-1 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
The anti-MAP3K20 antibody (A00902-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-05
Question
Is a blocking peptide available for product anti-MAP3K20 antibody (A00902-1)?
L. Dhar
Verified customer
Asked: 2016-04-08
Answer
We do provide the blocking peptide for product anti-MAP3K20 antibody (A00902-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-04-08
