Anti-MAPK3 MAPKAPK3 Antibody

Product Name

Anti-MAPK3 MAPKAPK3 Antibody

View all MAPKAP Kinase 3 Antibodies

SKU/Catalog Number

A04510

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MAPK3 MAPKAPK3 Antibody (Catalog # A04510). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAPK3 MAPKAPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04510)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MAPK3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04510 is reactive to MAPKAPK3 in Human, Mouse, Rat

Applications

A04510 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.987kDa

Background of MAPKAP Kinase 3

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.

McLaughlin M.M., J. Biol. Chem. 271:8488-8492(1996).
Sithanandam G., Mol. Cell. Biol. 16:868-876(1996).
Neufeld B., J. Biol. Chem. 275:20239-20242(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from COLO205 cells, using MAPK3 antibody A04510.

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using MAPK3 antibody A04510.

Gene/Protein Information For MAPKAPK3 (Source: Uniprot.org, NCBI)

Alternative Names

3PKEC 2.7.11.1; Chromosome 3p kinase; EC 2.7.11; MAP kinase-activated protein kinase 3; MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP33pK; MAPKAPK-3; mitogen-activated protein kinase-activated protein kinase 3 MAPKAPK3 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MDPT3, MK-3, MK3 MAPK activated protein kinase 3 MAP kinase-activated protein kinase 3|MAPKAP kinase 3|chromosome 3p kinase|mitogen-activated protein kinase-activated protein kinase 3

For more info on MAPKAPK3, check out the MAPKAPK3 Infographic

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In this infographic, you will see the following information for MAPKAPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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