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Product Info Summary
| SKU: | A04199 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ZC3HAV1 Antibody
SKU/Catalog Number
A04199
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZC3HAV1 Antibody (Catalog # A04199). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
ZC3HAV1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZC3HAV1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04199)
Host
Rabbit
Contents
ZC3HAV1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZC3HAV1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human ZC3HAV1. The immunogen is located within amino acids 680 - 730 of ZC3HAV1.
Reactive Species
A04199 is reactive to ZC3HAV1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
101.4 kDa
Background of ZC3HAV1
The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04199 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZC3HAV1 antibody can be used for detection of ZC3HAV1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.
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Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.
Specific Publications For Anti-ZC3HAV1 Antibody (A04199)
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