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Product Info Summary
| SKU: | A30663 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MARCH5 MARCH5; Antibody
SKU/Catalog Number
A30663
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MARCH5 MARCH5; Antibody (Catalog# A30663). Tested in ICC, IF, WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARCH5 MARCH5; Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30663)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MARCH5. Immunogen sequence location: 21-70
Reactive Species
A30663 is reactive to MARCH5; in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
31.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30663 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1,MARCH5 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MARCH5 MARCH5; Antibody (A30663)
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Customer Q&As
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1 Customer Q&As for Anti-MARCH5 MARCH5; Antibody
Question
Our lab want to know about using your anti-MARCH5 antibody for regulation of mitochondrial fission studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
J. Anderson
Verified customer
Asked: 2015-01-30
Answer
We appreciate your inquiry. This A30663 anti-MARCH5 antibody is tested on a549 cells. It is guaranteed to work for IF in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-30
