Click image to see more details
Product Info Summary
| SKU: | A04672-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-MARK4 Antibody
SKU/Catalog Number
A04672-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MARK4 Antibody (Catalog# A04672-1). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04672-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MARK4. Immunogen sequence location: 461-510
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04672-1 is reactive to MARK4 in Human, Mouse
Applications
A04672-1 is guaranteed for ELISA, IF, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
82.52kDa
Background of MARK4
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using MARK4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For MARK4 (Source: Uniprot.org, NCBI)
Gene Name
MARK4
Full Name
MAP/microtubule affinity-regulating kinase 4
Weight
82.52kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; FLJ12177; FLJ90097; KIAA1860MARK4L; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase like 1; MAP/microtubule affinity-regulating kinase-like 1; MARK4 serine/threonine protein kinase; MARKL1L; MARKL1MARK4S; Nbla00650 MARK4 MARK4LS, MARKL1, MARKL1L, PAR-1D, MARK4 microtubule affinity regulating kinase 4 MAP/microtubule affinity-regulating kinase 4|MAP/microtubule affinity-regulating kinase like 1|MARK4 serine/threonine protein kinase|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MARK4, check out the MARK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MARK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MARK4 Antibody (A04672-1)
Hello CJ!
No publications found for A04672-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
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- Scientific Literature Resources
- Research Paper Management Tools
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- Phylogenetic Tree Analysis
Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-MARK4 Antibody
Question
Will anti-MARK4 antibody A04672-1 work for IF with thyroid?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
According to the expression profile of thyroid, MARK4 is highly expressed in thyroid. So, it is likely that anti-MARK4 antibody A04672-1 will work for IF with thyroid.
Boster Scientific Support
Answered: 2020-04-20
Question
My question regarding product A04672-1, anti-MARK4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04672-1 anti-MARK4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-MARK4 antibody A04672-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The anti-MARK4 antibody (A04672-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-27
Question
Does anti-MARK4 antibody A04672-1 work on goat IF with male germ cell?
Z. Patel
Verified customer
Asked: 2019-10-14
Answer
Our lab technicians have not validated anti-MARK4 antibody A04672-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MARK4 antibody A04672-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat male germ cell in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid using anti-MARK4 antibody A04672-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
Please see the WB image, lot number and protocol we used for thyroid using anti-MARK4 antibody A04672-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-13
Question
Is a blocking peptide available for product anti-MARK4 antibody (A04672-1)?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
We do provide the blocking peptide for product anti-MARK4 antibody (A04672-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-08
Question
Is this A04672-1 anti-MARK4 antibody reactive to the isotypes of MARK4?
M. Parker
Verified customer
Asked: 2018-08-24
Answer
The immunogen of A04672-1 anti-MARK4 antibody is A peptide derived from human MARK4. Immunogen sequence location: 461-510. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-24
Question
I see that the anti-MARK4 antibody A04672-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-03
Question
I was wanting to use to test anti-MARK4 antibody A04672-1 on mouse thyroid for research purposes, then I may be interested in using anti-MARK4 antibody A04672-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Johnson
Verified customer
Asked: 2017-11-13
Answer
The products we sell, including anti-MARK4 antibody A04672-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-13
Question
I was wanting to use your anti-MARK4 antibody for IF for mouse thyroid on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thyroid identification?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
As indicated on the product datasheet, A04672-1 anti-MARK4 antibody has been validated for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse thyroid in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-12
Question
Is there a BSA free version of anti-MARK4 antibody A04672-1 available?
M. Williams
Verified customer
Asked: 2016-02-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MARK4 antibody A04672-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-23
Question
Would A04672-1 anti-MARK4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Thomas
Verified customer
Asked: 2014-07-21
Answer
As indicated on the product datasheet, A04672-1 anti-MARK4 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-21
