Anti-MARS2 Antibody Picoband™

Product Name

Anti-MARS2 Antibody Picoband™

View all Methionyl tRNA synthetase 2 Antibodies

SKU/Catalog Number

A10675

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MARS2 Antibody Picoband™ catalog # A10675. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MARS2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10675)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MARS2 recombinant protein (Position: H143-A590).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10675 is reactive to MARS2 in Human, Mouse, Rat

Applications

A10675 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

66.591kDa

Background of Methionyl tRNA synthetase 2

This gene produces a mitochondrial methionyl-tRNA synthetase protein that is encoded by the nuclear genome and imported to the mitochondrion. This protein likely functions as a monomer and is predicted to localize to the mitochondrial matrix. Mutations in this gene are associated with the autosomal recessive neurodegenerative disease spastic ataxia-3 (SPAX3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MARS2 using anti-MARS2 antibody (A10675).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse Raw264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARS2 antigen affinity purified polyclonal antibody (Catalog # A10675) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARS2 at approximately 70 kDa. The expected band size for MARS2 is at 70 kDa.

Gene/Protein Information For MARS2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 6.1.1; EC 6.1.1.10; methionine tRNA ligase 2, mitochondrial; Methionine--tRNA ligase 2; methionine-tRNA ligase; methionine-tRNA synthetase 2 (mitochondrial); methionine-tRNA synthetase 2, mitochondrial; methionyl-tRNA synthetase 2, mitochondrial; methionyl-tRNA synthetase, mitochondrial; MetRS; Mitochondrial methionine--tRNA ligase; mitochondrial methionyl-tRNA synthetase; mtMetRS MARS2 COXPD25, MetRS, mtMetRS methionyl-tRNA synthetase 2, mitochondrial methionine--tRNA ligase, mitochondrial|methionine tRNA ligase 2, mitochondrial|methionine--tRNA ligase 2|methionine-tRNA synthetase 2, mitochondrial

For more info on MARS2, check out the MARS2 Infographic

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In this infographic, you will see the following information for MARS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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