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- Table of Contents
Ca4 antibody (A01523-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Neuro-2a cells using anti-Ca4 antibody (A01523-3).
Overlay histogram showing Neuro-2a cells stained with A01523-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ca4 Antibody (A01523-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Neuro-2a cells stained with A01523-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ca4 Antibody (A01523-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for CA4? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CA4 Western Blot GuideBundle Save
Anti-Carbonic Anhydrase 4/CA4 Picoband® Antibody
Ca4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Ca4 antibody was positively detected by WB in:
mouse lung tissue, rat PC-12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ca4 antibody was positively detected by IHC in:
mouse kidney tissue, rat kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Ca4 antibody was positively detected by FC in:
mouse spleen cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
FC
Mouse
Tested
Tested
Recommend
Recommend
Tested
Rat
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Carbonic Anhydrase 4/CA4 Picoband® Antibody
View all CA4 Antibodies
View all CA4 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Carbonic Anhydrase 4/CA4 recombinant protein (Position: E25-L291).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
34.4 kDa
Background of Ca4
Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. It is mapped to 11; 11 C. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport.
Cite This Product
Anti-Carbonic Anhydrase 4/CA4 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01523-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
72%
Moderate Homology
Rabbit
57%
Low homology
Dog
57%
Low homology
Monkey
57%
Low homology
Human
56%
Low homology
Cow
55%
Low homology
Sheep
55%
Low homology
Zebrafish
42%
Low homology
Drosophila
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Ca4 antibody
Immunohistochemistry (IHC) protocol for Ca4 antibody
Flow Cytometry (FC) protocol for Ca4 antibody
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Product Promise
We promise Anti-Carbonic Anhydrase 4/CA4 Picoband® Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01523-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026