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Product Info Summary
| SKU: | A02093-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Matriptase 2/TMPRSS6 Antibody Picoband®
SKU/Catalog Number
A02093-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Matriptase 2/TMPRSS6 Antibody Picoband® catalog # A02093-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Matriptase 2/TMPRSS6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02093-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Matriptase 2/TMPRSS6 recombinant protein (Position: M1-Q759).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02093-1 is reactive to TMPRSS6 in Human, Mouse, Rat
Observed Molecular Weight
90 kDa, 95 kDa
Calculated molecular weight
90.0 kDa
Background of TMPRSS6
Transmembrane protease, serine 6 (also known as matriptase-2) is an enzyme that in humans is encoded by the TMPRSS6 gene. The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02093-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HCCT tissue, rat brain tissue, mouse brain tissue, rat liver tissue, mouse liver tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of Matriptase 2/TMPRSS6 using anti-Matriptase 2/TMPRSS6 antibody (A02093-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Matriptase 2/TMPRSS6 antigen affinity purified polyclonal antibody (Catalog # A02093-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Matriptase 2/TMPRSS6 at approximately 90,95kDa. The expected band size for Matriptase 2/TMPRSS6 is at 90 kDa.
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Flow Cytometry analysis of MCF-7 cells using anti-Matriptase 2/TMPRSS6 antibody (A02093-1).
Overlay histogram showing MCF-7 cells stained with A02093-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Matriptase 2/TMPRSS6 Antibody (A02093-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Matriptase 2/TMPRSS6 Antibody Picoband® (A02093-1)
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