Anti-MC1 Receptor/MC1R Antibody Picoband™

Product Name

Anti-MC1 Receptor/MC1R Antibody Picoband™

View all Melanocortin-1 R/MC1R Antibodies

SKU/Catalog Number

A00855-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MC1 Receptor/MC1R Antibody Picoband™ catalog # A00855-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MC1 Receptor/MC1R Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00855-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MC1 Receptor.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00855-1 is reactive to MC1R in Human, Mouse, Rat

Applications

A00855-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

34.706kDa

Background of Melanocortin-1 R/MC1R

The melanocortin 1 receptor (MC1R), mapped to 16q24.3, is also known as MSHR. This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MC1 using anti-MC1 antibody (A00855-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: PC12 whole cell lysates,
Lane 2: HEPA1-6 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: A375 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC1 antigen affinity purified polyclonal antibody (Catalog # A00855-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC1 at approximately 35KD. The expected band size for MC1 is at 35KD.

Gene/Protein Information For MC1R (Source: Uniprot.org, NCBI)

Alternative Names

CMM5; MC1R; MC1-R; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; Melanocortin-1 R; Melanocortin1R; Melanocortin-1R; melanocyte-stimulating hormone receptor; melanotropin receptor; MSHR; MSH-R; Mshra; MSH-RMGC14337; SHEP2; Tob MC1R CMM5, MSH-R, SHEP2 melanocortin 1 receptor melanocyte-stimulating hormone receptor|MC1-R|alpha melanocyte stimulating hormone receptor|melanotropin receptor

For more info on MC1R, check out the MC1R Infographic

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In this infographic, you will see the following information for MC1R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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