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Product Info Summary
| SKU: | A07019-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MC5R Antibody Picoband®
SKU/Catalog Number
A07019-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MC5R Antibody Picoband® catalog # A07019-3. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MC5R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07019-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse MC5R. Mouse MC5R shares 95.5% amino acid (aa) sequence identity with both human and rat MC5R.
Reactive Species
A07019-3 is reactive to MC5R in Mouse, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.0 kDa
Background of MC5R
Predicted to enable hormone binding activity and melanocortin receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway and regulation of metabolic process. Predicted to be active in cytoplasm and plasma membrane. Is expressed in skin. Orthologous to human MC5R (melanocortin 5 receptor).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07019-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Validation Images & Assay Conditions
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Western blot analysis of MC5R using anti-MC5R antibody (A07019-3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC5R antigen affinity purified polyclonal antibody (A07019-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MC5R at approximately 37 kDa. The expected band size for MC5R is at 37 kDa.
Specific Publications For Anti-MC5R Antibody Picoband® (A07019-3)
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