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Product Info Summary
| SKU: | A08501-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-MCMBP Antibody Picoband®
SKU/Catalog Number
A08501-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCMBP Antibody Picoband® catalog # A08501-1. Tested in WB, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MCMBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08501-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MCMBP recombinant protein (Position: E234-E534). Human MCMBP shares 94.7% and 95% amino acid (aa) sequence identity with mouse and rat MCMBP, respectively.
Reactive Species
A08501-1 is reactive to MCMBP in Human, Mouse, Rat
Calculated molecular weight
73.0 kDa
Background of MCMBP
This gene encodes a protein which is a component of the hexameric minichromosome maintenance (MCM) complex which regulates initiation and elongation of DNA. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08501-1 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunoprecipitation, 2-4 μg/ml, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of MCMBP using anti-MCMBP antibody (A08501-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCMBP antigen affinity purified polyclonal antibody (A08501-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCMBP at approximately 73 kDa. The expected band size for MCMBP is at 73 kDa.
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Immunoprecipitating MCMBP in 293T whole cell lysate.
Western blot analysis of MCMBP using anti-MCMBP antibody (A08501-1).
Lane 1: 293T whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-MCMBP antibody in 293T whole cell lysate,
Lane 3: anti-MCMBP antibody (2μg) + 293T whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCMBP antigen affinity purified polyclonal antibody (A08501-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MCMBP at approximately 73 kDa. The expected band size for MCMBP is at 73 kDa.
Specific Publications For Anti-MCMBP Antibody Picoband® (A08501-1)
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