Anti-MCP2/CCL8 Antibody Picoband™

Product Name

Anti-MCP2/CCL8 Antibody Picoband™

View all CCL8/MCP-2 Antibodies

SKU/Catalog Number

A03237-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCP2/CCL8 Antibody Picoband™ catalog # A03237-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MCP2/CCL8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03237-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MCP2/CCL8 recombinant protein (Position: Q24-P99).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03237-2 is reactive to CCL8 in Human, Mouse, Rat

Applications

A03237-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

11.246kDa

Background of CCL8/MCP-2

Chemokine (C-C motif) ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP2), is a protein that in humans is encoded by the CCL8 gene. This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MCP2/CCL8 using anti-MCP2/CCL8 antibody (A03237-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat small intestine tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP2/CCL8 antigen affinity purified polyclonal antibody (Catalog # A03237-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP2/CCL8 at approximately 22 kDa. The expected band size for MCP2/CCL8 is at 11 kDa.

Gene/Protein Information For CCL8 (Source: Uniprot.org, NCBI)

Alternative Names

C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8 CCL8 HC14, MCP-2, MCP2, SCYA10, SCYA8 C-C motif chemokine ligand 8 C-C motif chemokine 8|chemokine (C-C motif) ligand 8|monocyte chemoattractant protein 2|monocyte chemotactic protein 2|small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)|small-inducible cytokine A8

For more info on CCL8, check out the CCL8 Infographic

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In this infographic, you will see the following information for CCL8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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