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Product Info Summary
| SKU: | PB9029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MCP3/CCL7 Antibody Picoband®
SKU/Catalog Number
PB9029
PB0068 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCP3/CCL7 Antibody Picoband® catalog # PB9029. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCP3/CCL7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9029)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse MCP3 recombinant protein (Position: Q24-P97). Mouse MCP3 shares 60% and 95% amino acid (aa) sequences identity with human and rat MCP3, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9029 is reactive to Ccl7 in Mouse
Observed Molecular Weight
36 kDa
Calculated molecular weight
11.0 kDa
Background of Ccl7
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, MCP3 gene is mapped to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, it has been found that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9029 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, -
ELISA, 0.1-0.5μg/ml, -
Validation Images & Assay Conditions
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Western blot analysis of MCP3 using anti-MCP3 antibody (PB9029).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Mouse MCP3 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP3 antigen affinity purified polyclonal antibody (Catalog # PB9029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP3 at approximately 36KD. The expected band size for MCP3 is at 36KD.
Specific Publications For Anti-MCP3/CCL7 Antibody Picoband® (PB9029)
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1 Customer Q&As for Anti-MCP3/CCL7 Antibody Picoband®
Question
We are currently using anti-MCP3/CCL7 antibody PB9029 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-MCP3/CCL7 antibody (PB9029) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
