Anti-MCP4/CCL13 Antibody Picoband™

Product Name

Anti-MCP4/CCL13 Antibody Picoband™

View all CCL13/MCP-4 Antibodies

SKU/Catalog Number

PB9030

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCP4/CCL13 Antibody Picoband™ catalog # PB9030. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCP4/CCL13 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9030)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MCP4 recombinant protein (Position: Q24-T98).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9030 is reactive to CCL13 in Human

Applications

PB9030 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

10.986kDa

Background of CCL13/MCP-4

Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MCP4 using anti-MCP4 antibody (PB9030).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human MCP4 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP4 antigen affinity purified polyclonal antibody (Catalog # PB9030) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP4 at approximately 34KD. The expected band size for MCP4 is at 34KD.

Gene/Protein Information For CCL13 (Source: Uniprot.org, NCBI)

Alternative Names

C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13 CCL13 CKb10, MCP-4, NCC-1, NCC1, SCYA13, SCYL1 C-C motif chemokine ligand 13 C-C motif chemokine 13|CK-beta-10|chemokine (C-C motif) ligand 13|monocyte chemoattractant protein 4|monocyte chemotactic protein 4|new CC chemokine 1|small inducible cytokine subfamily A (Cys-Cys), member 13|small-inducible cytokine A13

For more info on CCL13, check out the CCL13 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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