Anti-MDA5 Monoclonal Antibody

Product Name

Anti-MDA5 Monoclonal Antibody

View all MDA5 Antibodies

SKU/Catalog Number

M00263-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MDA5 Monoclonal Antibody catalog # M00263-1. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MDA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00263-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADDD-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MDA5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00263-1 is reactive to IFIH1 in Human

Applications

M00263-1 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

116.689kDa

Background of MDA5

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of MDA5 expression in THP1 cell lysate.

Gene/Protein Information For IFIH1 (Source: Uniprot.org, NCBI)

Alternative Names

CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; EC 3.6.4.13; helicard; Helicase with 2 CARD domains; Hlcd; IDDM19; interferon induced with helicase C domain 1; interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5MGC133047; MDA5RNA helicase-DEAD box protein 116; melanoma differentiation associated protein-5; Melanoma differentiation-associated protein 5; Murabutide down-regulated protein; RH116 IFIH1 AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1 interferon induced with helicase C domain 1 interferon-induced helicase C domain-containing protein 1|CADM-140 autoantigen|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RIG-I-like receptor 2|RNA helicase-DEAD box protein 116|clinically amyopathic dermatomyositis autoantigen 140 kDa|helicard|helicase with 2 CARD domains|melanoma differentiation-associated gene 5|melanoma differentiation-associated protein 5|murabutide down-regulated protein

For more info on IFIH1, check out the IFIH1 Infographic

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In this infographic, you will see the following information for IFIH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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