Anti-MDMX/MDM4 Antibody Picoband™

Product Name

Anti-MDMX/MDM4 Antibody Picoband™

View all MDMX Antibodies

SKU/Catalog Number

A01889-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MDMX/MDM4 Antibody Picoband™ catalog # A01889-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MDMX/MDM4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01889-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MDMX/MDM4 recombinant protein (Position: A8-A490).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01889-2 is reactive to MDM4 in Human

Applications

A01889-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

54.864kDa

Background of MDMX

Protein Mdm4?is a?protein?that in humans is encoded by the?MDM4?gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MDMX/MDM4 using anti-MDMX/MDM4 antibody (A01889-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDMX/MDM4 antigen affinity purified polyclonal antibody (Catalog # A01889-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDMX/MDM4 at approximately 55 kDa. The expected band size for MDMX/MDM4 is at 55 kDa.

Gene/Protein Information For MDM4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp781B1423; double minute 4 homolog; Double minute 4 protein; double minute 4, human homolog of; p53-binding protein; HDMX; Mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog (mouse); MDM4 Regulator of P53; MDM4; Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse); Mdm4, transformed 3T3 cell double minute 4, p53 binding protein; MDM4-related protein 1; MDMX; MDMXMGC132766; mouse double minute 4, human homolog of; p53-binding protein; MRP1; p53-binding protein Mdm4; protein Mdm4; Protein Mdmx MDM4 BMFS6, HDMX, MDMX, MRP1 MDM4 regulator of p53 protein Mdm4|MDM4 protein variant G|MDM4 protein variant Y|MDM4, p53 regulator|MDM4-related protein 1|Mdm4 p53 binding protein homolog|double minute 4, human homolog of; p53-binding protein|mdm2-like p53-binding protein|protein Mdmx

For more info on MDM4, check out the MDM4 Infographic

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In this infographic, you will see the following information for MDM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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