Anti-MED1/Trap220 Antibody

Product Name

Anti-MED1/Trap220 Antibody

View all TRAP220/MED1 Antibodies

SKU/Catalog Number

A00550

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MED1/Trap220 Antibody (Catalog # A00550). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED1/Trap220 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00550)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MED1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00550 is reactive to MED1 in Human, Mouse

Applications

A00550 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

168.478kDa

Background of TRAP220/MED1

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Drane P., Oncogene 15:3013-3024(1997).
Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
Naeaer A.M., Nature 398:828-832(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from Jurkat cells, using MED1 antibody A00550.

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MED1 antibody A00550.

Gene/Protein Information For MED1 (Source: Uniprot.org, NCBI)

Alternative Names

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1TRIP2; CRSP200p53 regulatory protein RB18A; DRIP230; DRIP230PPAR-binding protein; MBD4; MED1; mediator complex subunit 1DRIP205; mediator of RNA polymerase II transcription subunit 1; PBPPPARGBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPARBP; PPARBPMGC71488; PPARG binding protein; PPARGBP; RB18ATR-interacting protein 2; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting pro MED1 CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2 mediator complex subunit 1 mediator of RNA polymerase II transcription subunit 1|ARC205|PPAR-binding protein|PPARG binding protein|TR-interacting protein 2|TRIP-2|activator-recruited cofactor 205 kDa component|p53 regulatory protein RB18A|peroxisome proliferator-activated receptor-binding protein|thyroid hormone receptor-associated protein complex 220 kDa component|thyroid hormone receptor-associated protein complex component TRAP220|thyroid receptor interacting protein 2|vitamin D receptor-interacting protein 230 kD|vitamin D receptor-interacting protein complex component DRIP205

For more info on MED1, check out the MED1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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